DNA

Part:BBa_K2100039:Design

Designed by: Wangui Mbuguiro   Group: iGEM16_MIT   (2016-10-17)


pEXPR pBM3R1 - eYFP


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 242
    Illegal PstI site found at 481
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 242
    Illegal NheI site found at 115
    Illegal PstI site found at 481
    Illegal NotI site found at 4
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 242
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 242
    Illegal PstI site found at 481
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 242
    Illegal PstI site found at 481
    Illegal AgeI site found at 264
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 108
    Illegal BsaI site found at 235


Design Notes

This composite part expression vector was created by an LR reaction. It is a promoter (flanked by L4, R1 sites) and a gene (flanked by L1, L2 sites) cloned into a backbone that has a negative selection marker between R4 and R2 sites. This part adheres to RFC 65 for recombination based cloning of mammalian parts.


Source

mammalian genomic sequence.

References